Description

PE-22-28 Peptide for Advanced TREK-1 Channel and Neuroscience Research

PE-22-28 Peptide is a synthetic analog derived from spadin, an endogenous peptide generated from the propeptide region of sortilin (SORT1). It was developed to selectively inhibit the TREK-1 (KCNK2) two-pore domain potassium channel, a membrane protein that plays a central role in regulating neuronal resting membrane potential, cellular excitability, mechanosensation, and neuronal adaptation.

Scientific investigations have established PE-22-28 as an important research reagent for examining potassium channel pharmacology, neuronal signaling, synaptic communication, neuroplasticity, and electrophysiological regulation. Laboratory studies continue to explore its effects on membrane depolarization, serotonergic neuron activity, intracellular calcium dynamics, transcriptional regulation, and signaling pathways associated with neuronal adaptation.

Beyond ion channel biology, PE-22-28 has become valuable in molecular neuroscience, peptide pharmacology, electrophysiology, and systems biology research. Its selective interaction with TREK-1 enables highly controlled experimental investigations of neuronal communication while minimizing off-target potassium channel activity.

Each batch is synthesized under stringent quality-controlled manufacturing procedures, analytically verified for purity and sequence identity, and supplied as a stable lyophilized powder with a comprehensive Certificate of Analysis to ensure highly reproducible laboratory research.

For research use only. Not intended for human consumption or clinical use.

Key Research Highlights

• Synthetic peptide derived from the endogenous spadin sequence
• Selective antagonist of the TREK-1 (KCNK2) potassium channel
• Extensively investigated in neuronal excitability research
• Frequently utilized in ion channel pharmacology studies
• Evaluated in synaptic plasticity and electrophysiology research
• Studied in serotonergic signaling and neuronal communication models
• Applied in neuroplasticity and molecular neuroscience investigations
• High-purity lyophilized peptide suitable for laboratory applications
• Certificate of Analysis (COA) supplied with every batch
• Manufactured under stringent analytical quality standards
• Excellent batch-to-batch reproducibility
• Suitable for biotechnology, pharmaceutical, and academic research laboratories

Why Researchers Choose This Product

• ≥99% research-grade purity verified through analytical testing
• Selective TREK-1 potassium channel inhibition
• Verified peptide identity and structural integrity
• Stable lyophilized formulation for laboratory storage
• Comprehensive Certificate of Analysis included
• Excellent reproducibility across experimental studies
• Suitable for neuroscience and electrophysiology research
• Manufactured using rigorous peptide synthesis protocols
• Available in multiple laboratory quantities
• Intended exclusively for scientific laboratory investigations

Research Applications

TREK-1 Ion Channel Research

PE-22-28 Peptide is widely utilized in laboratory investigations examining TREK-1 channel physiology, channel gating mechanisms, ion conductance, and potassium channel pharmacology.

Electrophysiology Studies

Researchers employ PE-22-28 to investigate neuronal membrane potential regulation, action potential generation, whole-cell patch clamp recordings, and neuronal excitability.

Molecular Neuroscience

Experimental models utilize PE-22-28 to study synaptic communication, intracellular signaling, neuroplasticity, neuronal adaptation, and transcriptional regulation within neural tissue.

Serotonergic Signaling Research

Scientists investigate neuronal signaling pathways associated with serotonergic neurons, neurotransmitter release, calcium-dependent signaling, and neurochemical communication.

Cellular Signal Transduction

PE-22-28 is incorporated into laboratory studies examining MAPK/ERK signaling, PI3K/Akt pathways, calcium signaling, CREB activation, and downstream transcriptional responses.

Peptide Pharmacology

Researchers utilize PE-22-28 for peptide stability studies, receptor-independent pharmacology, structure-function analysis, formulation development, and analytical characterization.

Product Specifications

Mechanism of Action

PE-22-28 Peptide is a synthetic analog derived from the endogenous sortilin propeptide fragment known as spadin and is primarily investigated as a selective inhibitor of the TWIK-related potassium channel-1 (TREK-1/KCNK2). TREK-1 belongs to the family of two-pore domain potassium (K2P) channels responsible for regulating background potassium conductance, resting membrane potential, and neuronal excitability. Because TREK-1 contributes to membrane hyperpolarization, selective inhibition with PE-22-28 provides researchers with an important tool for studying neuronal signaling under controlled laboratory conditions.

Published research demonstrates that PE-22-28 binds selectively to TREK-1 channels, reducing potassium efflux and promoting membrane depolarization. This alteration in membrane potential increases neuronal excitability and facilitates action potential generation in experimental neuronal systems. Researchers utilize these properties to investigate electrophysiological responses, synaptic communication, and mechanisms regulating neuronal network activity.

Laboratory investigations have further demonstrated downstream activation of intracellular signaling pathways associated with neuronal plasticity, including ERK1/2 (extracellular signal-regulated kinase), PI3K/Akt, and CREB (cAMP response element-binding protein) signaling. These molecular pathways regulate transcriptional activity, synaptic remodeling, neuronal differentiation, and intracellular communication. Additional studies have examined modulation of brain-derived neurotrophic factor (BDNF) expression and associated neurotrophic signaling networks following TREK-1 inhibition.

Experimental models have also explored interactions between PE-22-28 and serotonergic neuronal systems. By increasing excitability of serotonin-producing neurons, researchers investigate neurotransmitter release dynamics, neuronal firing patterns, calcium signaling, and peptide-mediated regulation of neurochemical communication. These observations remain confined to laboratory investigations and continue to advance understanding of ion channel biology and neuronal physiology.

Because of its high selectivity for TREK-1, reproducible biochemical characteristics, and well-defined mechanism of action, PE-22-28 Peptide has become a valuable research reagent for neuroscience, electrophysiology, ion channel pharmacology, molecular biology, peptide chemistry, and biotechnology laboratories investigating neuronal signaling and cellular communication.

For research use only. Not intended for human consumption or clinical use.